Photoprotective and Antioxidant Potential of Indonesia’s Klanceng Honey Beehive Waste
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Keywords:
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Antioxidant, Extract, Fraction, Honey Beehive, Photoprotective
Abstract
A byproduct of the honey processing called Klanceng honey beehive waste is said to still contain secondary metabolites that are beneficial to health. The objective of this research was to assess the photoprotective properties of an ethanol extract, n-hexane fraction, and ethyl acetate fraction obtained from the waste of an Indonesian Klanceng honey beehive that originated in Magelang. The DPPH free radical technique was applied to quantify antioxidant properties, and the Mansur equation’s SPF value was used to calculate photoprotective activity. The analysis of the data revealed that the IC50 values for the ethanol extract, n-hexane fraction, and ethyl acetate fraction were, respectively, 470.2935±0.9249, 207.1869± 2.6510, and 216.4892±0.8349. The ethanol extract, n-hexane fraction, and ethyl acetate fraction of the samples had SPF values of 3.872, 3.529, and 9.358, respectively. The ethyl acetate fraction, as opposed to the ethanol extract and the n-hexane fraction, has greater potential as a photoprotective agent as a result.
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Wagh, V. D. (2013). Propolis: A Wonder Bees Product and Its Pharmacological Potentials. Advances in Pharmacological Sciences, 2013; 1–13
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Birangane, R., D. Chole, and K. S. P. Reddy (2011). A Review of Antioxidants. Journal of Indian Academy of Oral Medicine and Radiology, 23(5); 351
Candiracci, M., E. Piatti, M. Dominguez Barragan, D. Garcia Antras, B. Morgado, D. Ruano, J. F. Gutierrez, J. Parrado, and A. Castano (2012). Anti-inflammatory Activity of A Honey Flavonoid Extract on Lipopolysaccharide-activated N13 Microglial Cells. Journal of Agricultural and Food Chemistry, 60(50); 12304–12311
Fernandez Garcia, E. (2014). Skin Protection Against UV Light by Dietary Antioxidants. Food & function, 5(9); 1994–2003
Geoffrey, K., A. Mwangi, and S. Maru (2019). Sunscreen Products: Rationale For Use, Formulation Development and Regulatory Considerations. Saudi Pharmaceutical Journal, 27(7); 1009–1018
Georgieva, K., M. Popova, L. Dimitrova, B. Trusheva, L. N. Thanh, D. T. L. Phuong, N. T. P. Lien, H. Najdenski, and V. Bankova (2019). Phytochemical Analysis of Vietnamese Propolis Produced by the Stingless Bee Lisotrigona Cacciae. PloS one, 14(4); e0216074
Gurning, K., S. Lumbangaol, R. F. Situmorang, and S. Silaban (2021). Determination of Phenolic Contents and Antioxidant Activity Test of Ethanol Extract of Sirih Merah (Piper Crocatum Ruiz & Pav.) Leaves Using The Dpph Method. Jurnal Pendidikan Kimia, 13(2); 137–142
Hrncir, M., S. Jarau, and F. G. Barth (2016). Stingless Bees (Meliponini): Senses and Behavior. Journal of Comparative Physiology A Neuroethology, Sensory, Neural, and Behavioral Physiology, 202(9); 597–601
Khan, M. A. (2018). Sun Protection Factor Determination Studies of some Sunscreen Formulations Used in Cosmetics for Their Selection. Journal of Drug Delivery and Therapeutics, 8(5-s); 149–151
Khoddami, A., M. A. Wilkes, and T. H. Roberts (2013). Techniques for Analysis of Plant Phenolic Compounds. Molecules, 18(2); 2328–2375
Kocot, J., M. Kiełczykowska, D. Luchowska Kocot, J. Kurzepa, and I. Musik (2018). Antioxidant Potential of Propolis, Bee Pollen, and Royal Jelly: Possible Medical Application. Oxidative Medicine and Cellular Longevity, 2018
Latha, M., J. Martis, V. Shobha, R. S. Shinde, S. Bangera, B. Krishnankutty, S. Bellary, S. Varughese, P. Rao, and B. N. Kumar (2013). Sunscreening Agents: A Review. The Journal of Clinical and Aesthetic Dermatology, 6(1); 16
Liu, H. x., S. q. Sun, G. h. Lv, and K. K. Chan (2006). Study on Angelica and Its Different Extracts by Fourier Transform Infrared Spectroscopy and Two-dimensional Correlation Ir Spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 64(2); 321–326
Mathur, R. and R. Vijayvergia (2017). Determination of Total Flavonoid and Phenol Content In Mimusops Elengi Linn. International Journal of Pharmaceutical Sciences and Research, 8(12); 5282–5285
Meilawati, L., T. Ernawati, R. T. Dewi, M. Megawati, and S. Sukirno (2021). Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice. Jurnal Kimia Terapan Indonesia, 23(2); 55–62
Molole, G. J., A. Gure, and N. Abdissa (2022). Determination of Total Phenolic Content and Antioxidant Activity of Commiphora mollis (Oliv.) Engl. Resin. BMC Chemistry,, 16(1); 1–11
Mouhoubi Tafinine, Z., S. Ouchemoukh, and A. Tamendjari (2016). Antioxydant Activity of Some Algerian Honey and Propolis. Industrial Crops and Products, 88; 85–90
Nurhasnawati, H., R. Sundu, S. Sapri, R. Supriningrum, H. Kuspradini, and E. T. Arung (2019). Antioxidant Activity, Total Phenolic and Flavonoid Content of Several Indigenous Species of Ferns In East Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 20(2); 576–580
Pérez-Pérez, E., E. Suárez, M. Peña Vera, A. González, and P. Vit (2013). Antioxidant Activity and Microorganisms In Nest Products of Tetragonisca Angustula Latreille, 1811 From Mérida, Venezuela. Stingless Bees Process Honey and Pollen In Cerumen Pots. Facultad De Farmacia Y Bioanálisis, Universidad De Los Andes; 1–8
Puspawati, G. A. K. D., Y. Marsono, S. Supriyadi, and R. Armunanto (2019). Comparison of Sonication With Maceration on Antioxidant Potency of Anthocyanin and Karotenoid of Tamarillo (Solanum Betaceaum Cav.). Agritech, 38(3); 304–312
Revathi, S. S. C. M. R., M. and C. M. Rigley (2019). Fourier Transform Infrared Spectroscopic Analysis Of EthanolicLeaf Extract Of Alternanthera Sessilis (L.) And Trigonella Foenum-Graecum (L.). Indian Journal Of Applied Research, 9(2); 45–46
Sabir, A. (2005). Aktivitas Antibakteri Flavonoid Propolis Trigona Sp Terhadap Bakteri Streptococcus Mutans (in Vitro)(in Vitro Antibacterial Activity Of Flavonoids Trigona Sp Propolis Against Streptococcus Mutans). Dental Journal (Majalah Kedokteran Gigi), 38(3); 135–141. (In Indonesia)
Sembiring, E. N., B. Elya, and R. Sauriasari (2018). Phytochemical Screening, Total Flavonoid and Total Phenolic Content and Antioxidant Activity of Different Parts of Caesalpinia Bonduc (l.) Roxb. Pharmacognosy Journal, 10(1)
Stuart, B. H. (2004). Infrared spectroscopy: fundamentals and applications. John Wiley & Sons Sukweenadhi, J., F. Setiawan, O. Yunita, K. Kartini, and
C. Avanti (2020). Antioxidant Activity Screening of Seven Indonesian Herbal Extract. Biodiversitas, 21(5); 2062–2067
Syarifah, S., E. Elfita, H. Widjajanti, A. Setiawan, and A. R. Kurniawati (2021). Diversity of Endophytic Fungi From The Root Bark Of Syzygium Zeylanicum, and The Antibacterial Activity of Fungal Extracts, and Secondary Metabolite. Biodiversitas Journal of Biological Diversity, 22(10)
Tristantini, D. and R. Amalia (2019). Quercetin Concentration and Total Flavonoid Content of Anti-atherosclerotic Herbs Using Aluminum Chloride Colorimetric Assay. AIP, 2193(1); 030012
Vijayakumar, R., S. S. Abd Gani, U. H. Zaidan, M. I. E. Halmi, T. Karunakaran, and M. R. Hamdan (2020). Exploring the Potential Use of Hylocereus Polyrhizus Peels As A Source of Cosmeceutical Sunscreen Agent For Its Antioxidant and Photoprotective Properties. Evidence Based Complementary and Alternative Medicine, 2020; 1–12
Wabaidur, S. M., M. S. Obbed, Z. A. Alothman, N. A. Alfaris, A. Y. Badjah Hadj Ahmed, M. R. Siddiqui, J. Z. Al tamimi, and T. S. Aldayel (2020). Total Phenolic Acids and Flavonoid Contents Determination in Yemeni Honey of Various Floral Sources: Folin-ciocalteu and Spectrophotometric Approach. Food Science and Technology, 40; 647–652
Wagh, V. D. (2013). Propolis: A Wonder Bees Product and Its Pharmacological Potentials. Advances in Pharmacological Sciences, 2013; 1–13
Yuan, G., Y. Guan, H. Yi, S. Lai, Y. Sun, and S. Cao (2021). Antibacterial Activity and Mechanism of Plant Flavonoids To Gram-positive Bacteria Predicted From Their Lipophilicities. Scientific Reports, 11(1); 1–1
Authors
Purwaningsih, Y., Masduqi, A. F., Indriyanti, E., & Syukur, M. (2023). Photoprotective and Antioxidant Potential of Indonesia’s Klanceng Honey Beehive Waste. Science and Technology Indonesia, 8(1), 137–143. https://doi.org/10.26554/sti.2023.8.1.137-143
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