Potential Analysis of Snakehead Fish Albumin Extract (Channa striata) as Immunostimulant and Anti-Inflammatory in Indomethacin-Induced Inflammatory Bowel Disease (IBD) Rats
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Keywords:
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Channa striata, Albumin, Oxidative Stress, Inflammation, Intestinal Barrier, Immunomodulation
Abstract
Inflammatory Bowel Disease (IBD) is a chronic gastrointestinal disorder driven by oxidative stress and immune dysregulation, often exacerbated by prolonged non-steroidal anti-inflammatory drug (NSAID) use. Conventional therapies provide symptomatic relief but are limited by severe adverse effects, necessitating safer alternatives. Channa striata (snakehead fish) is a rich source of albumin, essential amino acids, and bioactive compounds with potential immunomodulatory and anti-inflammatory effects. This study investigates the therapeutic efficacy of purified Channa striata albumin extract in an indomethacin-induced IBD rat model. Albumin was isolated via ammonium sulfate precipitation and characterized by SDS-PAGE, antioxidant activity (DPPH assay), and amino acid profiling. Rats were treated with Channa striata albumin (100-300 mg/kg BW) for 14 days post-indomethacin induction. Compared to controls and diclofenac-treated groups, the extract significantly reduced malondialdehyde (MDA) levels (up to 90.69%), improved jejunal histoarchitecture, and enhanced occludin expression. Immunohistochemistry showed a marked reduction in CD4+T cell infiltration, indicating immunomodulatory activity. These findings establish Channa striata albumin as a potent natural antioxidant and immunomodulator, offering a safer alternative to NSAIDs for IBD management.
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Dwijayanti, D. R., M. S. Djati, and M. Rifa’i (2016). The Role of VipAlbumin® as an Immunostimulatory Agent for Controlling Homeostasis and Proliferation of Lymphoid Cells. Central European Journal of Immunology, 1; 31–38.
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Fatma, N., M. Metusalach, N. A. Taslim, and M. Nurilmala (2023). SDS-PAGE Protein Profile of Albumin Extracted by Steaming from Four Marine and Three Brackish-Water Fishes. Biodiversitas Journal of Biological Diversity, 24(7); 4027–4033.
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Authors
Panjaitan, T. F. C. ., Fiddaroini, S., Suprayitno, E., Aulanni’am, A., & Hardoko, H. (2025). Potential Analysis of Snakehead Fish Albumin Extract (Channa striata) as Immunostimulant and Anti-Inflammatory in Indomethacin-Induced Inflammatory Bowel Disease (IBD) Rats. Science and Technology Indonesia, 10(3), 725–740. https://doi.org/10.26554/sti.2025.10.3.725-740
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