Structural Characterization and Immunogenic Evaluation of a 50 kDa Thyroid Peroxidase Fraction Isolated from Hypothyroid Patient Serum
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Keywords:
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Hypothyroidism, TPO, Autoimmune, CD4 T Expression, Immunohistochemistry
Abstract
Thyroid peroxidase (TPO) is a key enzyme involved in thyroid hormone biosynthesis and one of the major autoantigens in autoimmune thyroid disease. However, studies investigating immunoreactive serum-derived TPO-related protein fractions remain limited. This study aimed to isolate an approximately 50 kDa immunoreactive protein fraction from the serum of hypothyroid patients, characterize its biochemical properties, and evaluate its immunogenicity for rabbit polyclonal antibody production. Serum samples were initially characterized based on anti-TPO antibody levels and total protein concentration. Rabbits were immunized with the isolated protein, and the resulting immune response was evaluated by indirect ELISA, SDS-PAGE, Western blotting, histopathology, and immunohistochemistry. SDS-PAGE consistently revealed a distinct protein band at approximately 50 kDa. ATR-FTIR analysis demonstrated characteristic protein-associated functional groups, while LC-MS/MS confirmed the proteinaceous nature of the isolated fraction through amino acid composition analysis. Immunization induced a progressive increase in anti-TPO IgG levels, and the purified rabbit IgG exhibited the expected electrophoretic profile under reducing and non-reducing conditions. Western blot analysis demonstrated that the generated polyclonal antibodies recognized the isolated approximately 50 kDa protein fraction, whereas no signal was detected in the secondary antibody-only control. Histopathological and immunohistochemical analyses further supported activation of the immune response following immunization. These findings demonstrate that the isolated approximately 50 kDa immunoreactive protein fraction possesses biochemical and immunogenic properties and has potential as an experimental immunogen for polyclonal antibody production.
References
Abramson, J. and G. Anderson (2017). Thymic Epithelial Cells. Annual Review of Immunology, 35; 85–118
Akbar, R., H. Bashour, P. Rawat, P. A. Robert, E. Smorodina, T. S. Cotet, K. Flem-Karlsen, R. Frank, B. B. Mehta, M. H. Vu, T. Zengin, J. Gutierrez-Marcos, F. Lund-Johansen, J. T. Andersen, and V. Greiff (2022). Progress and Challenges for the Machine Learning-Based Design of Fit-for-Purpose Monoclonal Antibodies. mAbs, 14(1); 2008790
Ali, R. A. H., N. A. Mahana, A. A. H. Mahdi, D. S. Wahb, A. Bakr, and A. M. Badr (2025). Anti-TPO and Anti-TSHR Antibodies in Combination with T3, T4, and TSH Exhibited a Diagnostic Curve for Hashimoto Patients. The Journal of Basic and Applied Zoology, 86(1); 32
Anderson, N. L. and N. G. Anderson (2002). The Human Plasma Proteome: History, Character, and Diagnostic Prospects. Molecular & Cellular Proteomics, 1(11); 845–867
Antonelli, A., S. M. Ferrari, A. Corrado, A. Di Domenicantonio, and P. Fallahi (2015). Autoimmune Thyroid Disorders. Autoimmunity Reviews, 14(2); 174–180
Aulanni’am (2023). Protein dan Analisisnya. Inara Publisher, Malang, edisi revisi edition
Aulanni’am, A. B. Wijaya, D. K. Wuragil, A. P. W. Marhendra, A. D. Khairana, R. Rosandi, A. Rudijanto, and H. A. Tjahjono (2024). The Development of Monoclonal Antibody Against Thyroid-Stimulating Hormone for Congenital Hypothyroidism Screening in Indonesia. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 43(6); 153–159
Aulanni’am, D. K. Wuragil, D. W. Soeatmadji, Zulkarnain, and A. P. W. Marhendra (2018). Recombinant Protein Production from TPO Gen Cloning and Expression for Early Detection of Autoimmune Thyroid Diseases. IOP Conference Series: Materials Science and Engineering, 299(1); 012013
Azzahra, F. D., L. N. Mulyani, T. Sabrina, and N. A. Fithri (2025). Liposome Photosensitizer with Enzyme from Black Soybean Tempeh: Formula Optimization and In Vitro Thrombolytic Activity Evaluation. Science and Technology Indonesia, 10(3); 903–915
Benlaribi, R., Q. Gou, and H. Takaba (2022). Thymic Self-Antigen Expression for Immune Tolerance and Surveillance. Inflammation and Regeneration, 42(1); 28
Benvenga, S. and R. Vita (2018). Thyroid Peroxidase. In I. Huhtaniemi and L. Martini, editors, Encyclopedia of Endocrine Diseases. Elsevier, 2 edition, pages 433–438
Bossowski, A., M. Moniuszko, M. Dabrowska, M. Rusak, M. Jeznach, A. Bodzenta-Łukaszyk, and A. Bossowska (2013). Role of Th17 Cells and IL-17, IL-23 Cytokines in Pathogenesis of Autoimmune Thyroid Disease in Children. Thyroid Research, 6(Suppl 2); A8
Calsolaro, V., F. Niccolai, G. Pasqualetti, A. M. Calabrese, A. Polini, C. Okoye, S. Magno, N. Caraccio, and F. Monzani (2019). Overt and Subclinical Hypothyroidism in the Elderly: When to Treat? Frontiers in Endocrinology, 10(MAR); 177
Carter, M. J., R. M. Mitchell, P. M. Meyer Sauteur, D. F. Kelly, and J. Trück (2017). The Antibody-Secreting Cell Response to Infection: Kinetics and Clinical Applications. Frontiers in Immunology, 8; 630
Caturegli, P., A. De Remigis, and N. R. Rose (2014). Hashimoto Thyroiditis: Clinical and Diagnostic Criteria. Autoimmunity Reviews, 13(4–5); 391–397
Crotty, S. (2019). T Follicular Helper Cell Biology: A Decade of Discovery and Diseases. Immunity, 50(5); 1132–1148
Delahaut, P. (2017). Immunisation – Choice of Host, Adjuvants and Boosting Schedules with Emphasis on Polyclonal Antibody Production. Methods, 116; 4–11
Espenbetova, M., N. Kuzmina, A. Zubkov, V. Akhmetova, Z. Zamanbekova, A. Krykpaeva, Z. Zhumanbayeva, K. Amrenova, Z. Smailova, and N. Glushkova (2022). Epitopes Specificity of Antibodies to Thyroid Peroxidase in Patients with Graves’ Disease, Hashimoto’s Thyroiditis and Overlap-Syndrome. Journal of Clinical and Translational Endocrinology, 27; 100293
Ferrari, S. M., P. Fallahi, A. Antonelli, and S. Benvenga (2017). Environmental Issues in Thyroid Diseases. Frontiers in Endocrinology, 8; 50
Ferrari, S. M., F. Ragusa, G. Elia, S. R. Paparo, V. Mazzi, E. Baldini, S. Benvenga, A. Antonelli, and P. Fallahi (2021). Precision medicine in autoimmune thyroiditis and hypothyroidism. Frontiers in pharmacology, 12; 750380
Finkelstein, A. V. (2018). 50+ Years of Protein Folding. Biochemistry (Moscow), 83(Suppl. 1); S3–S18
Fiqriyana, M. A., Aulanni’am, and A. Roosdiana (2013). Characterization of Polyclonal Antibody Induced by Autoantibody TPO (Thyroidperoxidase) From Autoimmune Thyroid Disease (AITD) Serum with ELISA and Western Blotting. Journal of Pure and Applied Chemistry Research, 2(1); 19–26
Gessulat, S., T. Schmidt, D. P. Zolg, P. Samaras, K. Schnatbaum, J. Zerweck, T. Knaute, J. Rechenberger, B. Delanghe, A. Huhmer, U. Reimer, H. C. Ehrlich, S. Aiche, B. Kuster, and M. Wilhelm (2019). Prosit: Proteome-Wide Prediction of Peptide Tandem Mass Spectra by Deep Learning. Nature Methods, 16(6); 509–518
Geyer, P. E., L. M. Holdt, D. Teupser, and M. Mann (2017). Revisiting Biomarker Discovery by Plasma Proteomics. Molecular Systems Biology, 13(9); 942
Ghosh, R., J. E. Gilda, and A. V. Gomes (2014). The Necessity of and Strategies for Improving Confidence in the Accuracy of Western Blots. Expert Review of Proteomics, 11(5); 549–560
Godlewska, M., K. D. Arczewska, M. Rudzińska, A. Łyczkowska, W. Krasuska, K. Hanusek, J. Ruf, M. Kiedrowski, and B. Czarnocka (2017). Thyroid Peroxidase (TPO) Expressed in Thyroid and Breast Tissues Shows Similar Antigenic Properties. PLoS ONE, 12(6); e0179066
Godlewska, M., D. Gawel, A. M. Buckle, and J. P. Banga (2019). Thyroid Peroxidase Revisited—What’s New? Hormone and Metabolic Research, 51(12); 765–769
Greenfield, E. A. (2022). Immunizing Animals. Cold Spring Harbor Protocols, 2022(7); 267–294
Gulla, S., M. C. Reddy, V. C. Reddy, S. Chitta, M. Bhanoori, and D. Lomada (2023). Role of Thymus in Health and Disease. International Reviews of Immunology, 42(5); 347–363
Hammarlund, E., A. Thomas, I. J. Amanna, L. A. Holden, O. D. Slayden, B. Park, L. Gao, and M. K. Slifka (2017). Plasma Cell Survival in the Absence of B Cell Memory. Nature Communications, 8(1); 1781
Hato, L., A. Vizcay, I. Eguren, J. L. Pérez-Gracia, J. Rodríguez, J. Gállego Pérez-Larraya, P. Sarobe, S. Inogés, A. L. Díaz de Cerio, and M. Santisteban (2024). Dendritic Cells in Cancer Immunology and Immunotherapy. Cancers, 16(5); 981
Hempelmann, E. and K. Krafts (2017). The Mechanism of Silver Staining of Proteins Separated by SDS Polyacrylamide Gel Electrophoresis. Biotechnic & Histochemistry, 92(2); 79–85
Hirahara, K. and T. Nakayama (2016). CD4+ T-Cell Subsets in Inflammatory Diseases: Beyond the Th1/Th2 Paradigm. International Immunology, 28(4); 163–171
Hu, X., Y. Chen, Y. Shen, R. Tian, Y. Sheng, and H. Que (2022). Global Prevalence and Epidemiological Trends of Hashimoto’s Thyroiditis in Adults: A Systematic Review and Meta-Analysis. Frontiers in Public Health, 10; 1020707
Huang, L., X. Deng, R. Li, Y. Xia, G. Bai, K. H. M. Siddique, and P. Guo (2018). A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers Using a Non-Denaturing Polyacrylamide Gel. Journal of Visualized Experiments, (134); 57192
Jankovic, J., M. Dettwiler, M. G. Fernández, E. Tièche, K. Hahn, S. April-Monn, M. S. Dettmer, M. Kessler, S. Rottenberg, and M. Campos (2021). Validation of Immunohistochemistry for Canine Proteins Involved in Thyroid Iodine Uptake and Their Expression in Canine Follicular Cell Thyroid Carcinomas (FTCs) and FTC-Derived Organoids. Veterinary Pathology, 58(6); 1172–1180
Jin, Y., D. Shi, and L. Wang (2025). Application Value of Chemiluminescence Immunoassay in Thyroid Function Biochemical Testing. Journal of Fluorescence
Kaspar, H., K. Dettmer, W. Gronwald, and P. J. Oefner (2009). Advances in Amino Acid Analysis. Analytical and Bioanalytical Chemistry, 393(2); 445–452
Khairana, A. D., S. Fiddaroini, A. B. Wijaya, R. Rosandi, A. Sabarudin, A. Safitri, W. Riawan, D. K. Wuragil, and Aulanniam (2025). Computational Analysis of TSHb Variants (Native, Mutated and Deleted) in Interaction with TSHR: Molecular Docking and Dynamic Simulations for Mechanistic Insights into Congenital Hypothyroidism. Trends in Sciences, 22(8); 10086
Kirley, T. L. and A. B. Norman (2018). Unfolding of IgG Domains Detected by Non-Reducing SDS-PAGE. Biochemical and Biophysical Research Communications, 503(2); 944–949
Klein, L., E. A. Robey, and C.-S. Hsieh (2019). Central CD4+ T Cell Tolerance: Deletion Versus Regulatory T Cell Differentiation. Nature Reviews Immunology, 19(1); 7–18
Klubo-Gwiezdzinska, J. and L. Wartofsky (2022). Hashimoto Thyroiditis: An Evidence-Based Guide to Etiology, Diagnosis and Treatment. Polish Archives of Internal Medicine, 132(3); 16222
Le, S. N., B. T. Porebski, J. McCoey, J. Fodor, B. Riley, M. Godlewska, M. Góra, B. Czarnocka, J. P. Banga, D. E. Hoke, I. Kass, and A. M. Buckle (2015). Modelling of Thyroid Peroxidase Reveals Insights into Its Enzyme Function and Autoantigenicity. PLoS ONE, 10(12); e0142615
Lewis, S. M., A. Williams, and S. C. Eisenbarth (2019). Structure and Function of the Immune System in the Spleen. Science Immunology, 4(33); eaau6085
Liu, T., Y. Wang, X. Luo, J. Li, S. A. Reed, H. Xiao, T. S. Young, and P. G. Schultz (2016). Enhancing Protein Stability with Extended Disulfide Bonds. Proceedings of the National Academy of Sciences of the United States of America, 113(21); 5910–5915
Liu, Z., M. J. Tu, C. Zhang, J. L. Jilek, Q. Y. Zhang, and A.-M. Yu (2019). A Reliable LC-MS/MS Method for the Quantification of Natural Amino Acids in Mouse Plasma: Method Validation and Application to a Study on Amino Acid Dynamics During Hepatocellular Carcinoma Progression. Journal of Chromatography B, 1124; 72–81
Loi Julia, S.-H. and L.-H. Gam (2020). SDS-PAGE Protein Profiling of a Few Commonly Consumed Beans. Journal of Biotechnology and Bioengineering, 4(1)
Ma, S. G., Y. L. Qiu, H. Zhu, H. Liu, Q. Li, and C. M. Ji (2015). Novel Genetic Variants in the TPO Gene Cause Congenital Hypothyroidism. Scandinavian Journal of Clinical and Laboratory Investigation, 75(8); 633–637
Makaraviciute, A., C. D. Jackson, P. A. Millner, and A. Ramanaviciene (2016). Considerations in Producing Preferentially Reduced Half-Antibody Fragments. Journal of Immunological Methods, 429; 50–56
Mariam, S. H. S., R. Noorain, W. S. Tan, A. Arbakariya, and O. A. Janna (2026). Purification and Characterization of Rabbit Polyclonal Immunoglobulin G Using DEAE Ion-Exchange Chromatography and Dynamic Light Scattering. Protein Expression and Purification, 242; 106938
Marx, A., Y. Yamada, K. Simon-Keller, B. Schalke, N. Willcox, P. Ströbel, and C. A. Weis (2021). Thymus and Autoimmunity. Seminars in Immunopathology, 43(1); 45–64
McLachlan, S. M. and B. Rapoport (2017). Thyroid Autoantibodies Display Both “Original Antigenic Sin” and Epitope Spreading. Frontiers in Immunology, 8; 1845
McLeod, D. S. A. and D. S. Cooper (2012). The Incidence and Prevalence of Thyroid Autoimmunity. Endocrine, 42(2); 252–265
Miller, J. F. A. P. (2020). The Function of the Thymus and Its Impact on Modern Medicine. Science, 369(6503); eaba2429
Neto, V., S. Esteves-Ferreira, I. Inácio, M. Alves, R. Dantas, I. Almeida, J. Guimarães, T. Azevedo, and A. Nunes (2022). Metabolic Profile Characterization of Different Thyroid Nodules Using FTIR Spectroscopy: A Review. Metabolites, 12(1); 53
Noor, Z., S. B. Ahn, M. S. Baker, S. Ranganathan, and A. Mohamedali (2021). Mass Spectrometry-Based Protein Identification in Proteomics: A Review. Briefings in Bioinformatics, 22(2); 1620–1638
Nugroho, I. C., M. N. Amalia, M. R. Juniardi, E. B. Marhendra, N. A. Yanma, O. R. Adianingsih, S. Winarsih, N. Widodo, and V. Yurina (2026). Oral Immunization with Recombinant Lactococcus Lactis and Retinoic Acid Boost Immune Response in Mice. Science and Technology Indonesia, 11(1); 55–64
Panjaitan, T. F. C., S. Fiddaroini, E. Suprayitno, Aulanni’am, and Hardoko (2025). Potential Analysis of Snakehead Fish Albumin Extract (Channa striata) as Immunostimulant and Anti-Inflammatory in Indomethacin-Induced Inflammatory Bowel Disease (IBD) Rats. Science and Technology Indonesia, 10(3); 725–740
Patagar, P. M. and S. Rudrappa (2022). Autoimmune Thyroiditis in Adolescents: Two Different Presentations. Karnataka Paediatric Journal, 37; 30–34
Pedersen, I. B. and P. Laurberg (2009). Antibodies to Thyroid Peroxidase and Thyroglobulin in Iodine Deficiencies. In Comprehensive Handbook of Iodine: Nutritional, Biochemical, Pathological and Therapeutic Aspects. Elsevier, pages 575–585
Pushpagiri, N., L. Gracelyn, and S. Nagalingam (2015). Prevalence of Subclinical and Overt Hypothyroidism in Infertile Women. International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 4(6); 1733–1738
Ragusa, F., P. Fallahi, G. Elia, D. Gonnella, S. R. Paparo, C. Giusti, L. P. Churilov, S. M. Ferrari, and A. Antonelli (2019). Hashimoto’s Thyroiditis: Epidemiology, Pathogenesis, Clinic and Therapy. Best Practice & Research: Clinical Endocrinology & Metabolism, 33(6); 101367
Ramadani, D., A. B. Wijaya, A. Srihardyastutie, D. K. Wuragil, R. Rosandi, A. Rudijanto, and Aulanni’am (2024). Characterization of TPO Enzyme and Its Immunogenicity in New Zealand White Rabbits (Oryctolagus cuniculus). Jurnal Penelitian Pendidikan IPA, 10(12); 10645–10652
Raphael, I., R. R. Joern, and T. G. Forsthuber (2020). Memory CD4+ T Cells in Immunity and Autoimmune Diseases. Cells, 9(3); 531
Raphael, I., S. Nalawade, T. N. Eagar, and T. G. Forsthuber (2015). T Cell Subsets and Their Signature Cytokines in Autoimmune and Inflammatory Diseases. Cytokine, 74(1); 5–17
Rapoport, B. and S. M. McLachlan (2018). Reflections on Thyroid Autoimmunity: A Personal Overview from the Past into the Future. Hormone and Metabolic Research, 50(12); 840–852
Rehlund, A. S. G., T. S. Canoy, D. S. Nielsen, M. N. Lund, and M. M. Poojary (2025). Protein Hydrolysis for Amino Acid Analysis Revisited. Food Chemistry, 495; 146224
Rose, A., D. Funk, and R. Neiger (2016). Comparison of Refractometry and Biuret Assay for Measurement of Total Protein Concentration in Canine Abdominal and Pleural Fluid Specimens. Journal of the American Veterinary Medical Association, 248(7); 789–794
Rothschild, K. J. (2016). The Early Development and Application of FTIR Difference Spectroscopy to Membrane Proteins: A Personal Perspective. Biomedical Spectroscopy and Imaging, 5(3); 231–267
Ruterbusch, M., K. B. Pruner, L. Shehata, and M. Pepper (2020). In Vivo CD4+ T Cell Differentiation and Function: Revisiting the Th1/Th2 Paradigm. Annual Review of Immunology, 38; 705–725
Salman, M. A., A. Rabiee, A. Salman, and T. E. Ftohy (2020). Antithyroid Peroxidase Antibodies and Histopathological Outcomes in Egyptian Patients Subjected to Total Thyroidectomy for Nonmalignant Nodular Goiter. Research Square
Shawkat Kawther, R., R. Tahir Lak, and S. Mohammed Zaki Abdullah (2022). The Role of Anti-TPO as an Additional Analytical Marker in Thyroid Disease Patients in Erbil City. Diyala Journal of Medicine, 23(2); 120–130
Shehata, M., H. Saad Eldien, F. Meligy, and S. Bahaidarh (2019). The Possible Protective Role of Barley Seeds on the Spleen After Administration of Glucocorticoids in Adult Albino Rats: A Histological and Immunohistochemical Study. Journal of Microscopy and Ultrastructure, 7(4); 171–180
Siriwardhane, T., K. Krishna, V. Ranganathan, V. Jayaraman, T. Wang, K. Bei, S. Ashman, K. Rajasekaran, J. J. Rajasekaran, and H. Krishnamurthy (2019). Significance of Anti-TPO as an Early Predictive Marker in Thyroid Disease. Autoimmune Diseases, 2019; 1684074
Stebegg, M., S. D. Kumar, A. Silva-Cayetano, V. R. Fonseca, M. A. Linterman, and L. Graca (2018). Regulation of the Germinal Center Response. Frontiers in Immunology, 9; 2469
Suan, D., C. Sundling, and R. Brink (2017). Plasma Cell and Memory B Cell Differentiation from the Germinal Center. Current Opinion in Immunology, 45; 97–102
Takaba, H. and H. Takayanagi (2017). The Mechanisms of T Cell Selection in the Thymus. Trends in Immunology, 38(11); 805–816
Tater, A., A. Gupta, G. Upadhyay, A. Deshpande, R. Date, and I. Y. Tamboli (2021). In Vitro Assays for Characterization of Distinct Multiple Catalytic Activities of Thyroid Peroxidase Using LC-MS/MS. Current Research in Toxicology, 2; 19–29
Tatulian, S. A. (2019). FTIR Analysis of Proteins and Protein–Membrane Interactions. In Methods in Molecular Biology, volume 2003. Humana Press, pages 281–325
Teijaro, J. R., D. Turner, Q. Pham, E. J. Wherry, L. Lefrançois, and D. L. Farber (2011). Cutting Edge: Tissue-Retentive Lung Memory CD4 T Cells Mediate Optimal Protection to Respiratory Virus Infection. The Journal of Immunology, 187(11); 5510–5514
Tintor, K. Ninković, J. Milošević, and N. Polović (2024). Gaining Insight into Protein Structure via ATR-FTIR Spectroscopy. Vibrational Spectroscopy, 134; 103726
Trifanescu, R. A. and C. Poiana (2019). Diagnosis and Management of Hypothyroidism in Adults. In Advanced Practice in Endocrinology Nursing. Springer International Publishing, pages 581–592
Tuska, H. S. A., A. M. Hariati, A. Sanusi, G. Ciptadi, B. Rumhayati, H. Susanto, G. Residiwati, and Aulanni’am (2025). Reproductive Toxicity and Histopathological Effects of Heavy Metal Exposure in Male Nile Tilapia from the Brantas River, Indonesia. Biodiversitas, 26(10); 5169–5180
Uhlén, M., A. Bandrowski, S. Carr, A. Edwards, J. Ellenberg, E. Lundberg, D. L. Rimm, H. Rodriguez, T. Hiltke, M. Snyder, and T. Yamamoto (2016). A Proposal for Validation of Antibodies. Nature Methods, 13(10); 823–827
Valentine, K. M., D. Davini, T. J. Lawrence, G. N. Mullins, M. Manansala, M. Al-Kuhlani, J. M. Pinney, J. K. Davis, A. E. Beaudin, S. S. Sindi, D. M. Gravano, and K. K. Hoyer (2018). CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. The Journal of Immunology, 201(1); 31–40
Vancauwenberghe, T., A. Snoeckx, D. Vanbeckevoort, S. Dymarkowski, and F. M. Vanhoenacker (2015). Imaging of the Spleen: What the Clinician Needs to Know. Singapore Medical Journal, 56(3); 133–144
Villela, L. F. d. A., L. C. C. d. Freitas, M. Brandão, L. Bachmann, and R. Iwakura (2018). Characterization of Thyroid Tissue Using Infrared Spectroscopy. Archives of Head and Neck Surgery, 47(1); e0874
Wang, L., F.-S. Wang, and M. E. Gershwin (2015). Human Autoimmune Diseases: A Comprehensive Update. Journal of Internal Medicine, 278(4); 369–395
Weetman, A. P. (2021). An Update on the Pathogenesis of Hashimoto’s Thyroiditis. Journal of Endocrinological Investigation, 44(5); 883–890
Weisel, F. and M. Shlomchik (2017). Memory B Cells of Mice and Humans. Annual Review of Immunology, 35; 255–284
Williams, D. E., S. N. Le, D. E. Hoke, P. G. Chandler, M. Gora, M. Godlewska, J. P. Banga, and A. M. Buckle (2020). Structural Studies of Thyroid Peroxidase Show the Monomer Interacting with Autoantibodies in Thyroid Autoimmune Disease. Endocrinology, 161(2); bqaa016
Yadav, V., D. Dabar, A. D. Goel, M. Bairwa, A. Sood, P. Prasad, S. S. Agarwal, and S. Nandeshwar (2021). Prevalence of Hypothyroidism in Pregnant Women in India: A Meta-Analysis of Observational Studies. Journal of Thyroid Research, 2021; 5515831
Yamano, T., J. Nedjic, M. Hinterberger, M. Steinert, S. Koser, S. Pinto, N. Gerdes, E. Lutgens, N. Ishimaru, M. Busslinger, B. Brors, B. Kyewski, and L. Klein (2015). Thymic B Cells Are Licensed to Present Self Antigens for Central T Cell Tolerance Induction. Immunity, 42(6); 1048–1061
Zhang, C.-H., X.-J. Liu, Y.-F. Wang, J.-H. Lu, H.-Y. Zheng, S. Xiong, M.-Y. Zhang, and Q.-Y. Liu (2007). Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus. Virologica Sinica, 22(5); 425–431
Zhu, J. (2018). T Helper Cell Differentiation, Heterogeneity, and Plasticity. Cold Spring Harbor Perspectives in Biology, 10(10); a030338
Zhu, J., H. Yamane, and W. E. Paul (2010). Differentiation of Effector CD4+ T Cell Populations. Annual Review of Immunology, 28; 445–489
Akbar, R., H. Bashour, P. Rawat, P. A. Robert, E. Smorodina, T. S. Cotet, K. Flem-Karlsen, R. Frank, B. B. Mehta, M. H. Vu, T. Zengin, J. Gutierrez-Marcos, F. Lund-Johansen, J. T. Andersen, and V. Greiff (2022). Progress and Challenges for the Machine Learning-Based Design of Fit-for-Purpose Monoclonal Antibodies. mAbs, 14(1); 2008790
Ali, R. A. H., N. A. Mahana, A. A. H. Mahdi, D. S. Wahb, A. Bakr, and A. M. Badr (2025). Anti-TPO and Anti-TSHR Antibodies in Combination with T3, T4, and TSH Exhibited a Diagnostic Curve for Hashimoto Patients. The Journal of Basic and Applied Zoology, 86(1); 32
Anderson, N. L. and N. G. Anderson (2002). The Human Plasma Proteome: History, Character, and Diagnostic Prospects. Molecular & Cellular Proteomics, 1(11); 845–867
Antonelli, A., S. M. Ferrari, A. Corrado, A. Di Domenicantonio, and P. Fallahi (2015). Autoimmune Thyroid Disorders. Autoimmunity Reviews, 14(2); 174–180
Aulanni’am (2023). Protein dan Analisisnya. Inara Publisher, Malang, edisi revisi edition
Aulanni’am, A. B. Wijaya, D. K. Wuragil, A. P. W. Marhendra, A. D. Khairana, R. Rosandi, A. Rudijanto, and H. A. Tjahjono (2024). The Development of Monoclonal Antibody Against Thyroid-Stimulating Hormone for Congenital Hypothyroidism Screening in Indonesia. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 43(6); 153–159
Aulanni’am, D. K. Wuragil, D. W. Soeatmadji, Zulkarnain, and A. P. W. Marhendra (2018). Recombinant Protein Production from TPO Gen Cloning and Expression for Early Detection of Autoimmune Thyroid Diseases. IOP Conference Series: Materials Science and Engineering, 299(1); 012013
Azzahra, F. D., L. N. Mulyani, T. Sabrina, and N. A. Fithri (2025). Liposome Photosensitizer with Enzyme from Black Soybean Tempeh: Formula Optimization and In Vitro Thrombolytic Activity Evaluation. Science and Technology Indonesia, 10(3); 903–915
Benlaribi, R., Q. Gou, and H. Takaba (2022). Thymic Self-Antigen Expression for Immune Tolerance and Surveillance. Inflammation and Regeneration, 42(1); 28
Benvenga, S. and R. Vita (2018). Thyroid Peroxidase. In I. Huhtaniemi and L. Martini, editors, Encyclopedia of Endocrine Diseases. Elsevier, 2 edition, pages 433–438
Bossowski, A., M. Moniuszko, M. Dabrowska, M. Rusak, M. Jeznach, A. Bodzenta-Łukaszyk, and A. Bossowska (2013). Role of Th17 Cells and IL-17, IL-23 Cytokines in Pathogenesis of Autoimmune Thyroid Disease in Children. Thyroid Research, 6(Suppl 2); A8
Calsolaro, V., F. Niccolai, G. Pasqualetti, A. M. Calabrese, A. Polini, C. Okoye, S. Magno, N. Caraccio, and F. Monzani (2019). Overt and Subclinical Hypothyroidism in the Elderly: When to Treat? Frontiers in Endocrinology, 10(MAR); 177
Carter, M. J., R. M. Mitchell, P. M. Meyer Sauteur, D. F. Kelly, and J. Trück (2017). The Antibody-Secreting Cell Response to Infection: Kinetics and Clinical Applications. Frontiers in Immunology, 8; 630
Caturegli, P., A. De Remigis, and N. R. Rose (2014). Hashimoto Thyroiditis: Clinical and Diagnostic Criteria. Autoimmunity Reviews, 13(4–5); 391–397
Crotty, S. (2019). T Follicular Helper Cell Biology: A Decade of Discovery and Diseases. Immunity, 50(5); 1132–1148
Delahaut, P. (2017). Immunisation – Choice of Host, Adjuvants and Boosting Schedules with Emphasis on Polyclonal Antibody Production. Methods, 116; 4–11
Espenbetova, M., N. Kuzmina, A. Zubkov, V. Akhmetova, Z. Zamanbekova, A. Krykpaeva, Z. Zhumanbayeva, K. Amrenova, Z. Smailova, and N. Glushkova (2022). Epitopes Specificity of Antibodies to Thyroid Peroxidase in Patients with Graves’ Disease, Hashimoto’s Thyroiditis and Overlap-Syndrome. Journal of Clinical and Translational Endocrinology, 27; 100293
Ferrari, S. M., P. Fallahi, A. Antonelli, and S. Benvenga (2017). Environmental Issues in Thyroid Diseases. Frontiers in Endocrinology, 8; 50
Ferrari, S. M., F. Ragusa, G. Elia, S. R. Paparo, V. Mazzi, E. Baldini, S. Benvenga, A. Antonelli, and P. Fallahi (2021). Precision medicine in autoimmune thyroiditis and hypothyroidism. Frontiers in pharmacology, 12; 750380
Finkelstein, A. V. (2018). 50+ Years of Protein Folding. Biochemistry (Moscow), 83(Suppl. 1); S3–S18
Fiqriyana, M. A., Aulanni’am, and A. Roosdiana (2013). Characterization of Polyclonal Antibody Induced by Autoantibody TPO (Thyroidperoxidase) From Autoimmune Thyroid Disease (AITD) Serum with ELISA and Western Blotting. Journal of Pure and Applied Chemistry Research, 2(1); 19–26
Gessulat, S., T. Schmidt, D. P. Zolg, P. Samaras, K. Schnatbaum, J. Zerweck, T. Knaute, J. Rechenberger, B. Delanghe, A. Huhmer, U. Reimer, H. C. Ehrlich, S. Aiche, B. Kuster, and M. Wilhelm (2019). Prosit: Proteome-Wide Prediction of Peptide Tandem Mass Spectra by Deep Learning. Nature Methods, 16(6); 509–518
Geyer, P. E., L. M. Holdt, D. Teupser, and M. Mann (2017). Revisiting Biomarker Discovery by Plasma Proteomics. Molecular Systems Biology, 13(9); 942
Ghosh, R., J. E. Gilda, and A. V. Gomes (2014). The Necessity of and Strategies for Improving Confidence in the Accuracy of Western Blots. Expert Review of Proteomics, 11(5); 549–560
Godlewska, M., K. D. Arczewska, M. Rudzińska, A. Łyczkowska, W. Krasuska, K. Hanusek, J. Ruf, M. Kiedrowski, and B. Czarnocka (2017). Thyroid Peroxidase (TPO) Expressed in Thyroid and Breast Tissues Shows Similar Antigenic Properties. PLoS ONE, 12(6); e0179066
Godlewska, M., D. Gawel, A. M. Buckle, and J. P. Banga (2019). Thyroid Peroxidase Revisited—What’s New? Hormone and Metabolic Research, 51(12); 765–769
Greenfield, E. A. (2022). Immunizing Animals. Cold Spring Harbor Protocols, 2022(7); 267–294
Gulla, S., M. C. Reddy, V. C. Reddy, S. Chitta, M. Bhanoori, and D. Lomada (2023). Role of Thymus in Health and Disease. International Reviews of Immunology, 42(5); 347–363
Hammarlund, E., A. Thomas, I. J. Amanna, L. A. Holden, O. D. Slayden, B. Park, L. Gao, and M. K. Slifka (2017). Plasma Cell Survival in the Absence of B Cell Memory. Nature Communications, 8(1); 1781
Hato, L., A. Vizcay, I. Eguren, J. L. Pérez-Gracia, J. Rodríguez, J. Gállego Pérez-Larraya, P. Sarobe, S. Inogés, A. L. Díaz de Cerio, and M. Santisteban (2024). Dendritic Cells in Cancer Immunology and Immunotherapy. Cancers, 16(5); 981
Hempelmann, E. and K. Krafts (2017). The Mechanism of Silver Staining of Proteins Separated by SDS Polyacrylamide Gel Electrophoresis. Biotechnic & Histochemistry, 92(2); 79–85
Hirahara, K. and T. Nakayama (2016). CD4+ T-Cell Subsets in Inflammatory Diseases: Beyond the Th1/Th2 Paradigm. International Immunology, 28(4); 163–171
Hu, X., Y. Chen, Y. Shen, R. Tian, Y. Sheng, and H. Que (2022). Global Prevalence and Epidemiological Trends of Hashimoto’s Thyroiditis in Adults: A Systematic Review and Meta-Analysis. Frontiers in Public Health, 10; 1020707
Huang, L., X. Deng, R. Li, Y. Xia, G. Bai, K. H. M. Siddique, and P. Guo (2018). A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers Using a Non-Denaturing Polyacrylamide Gel. Journal of Visualized Experiments, (134); 57192
Jankovic, J., M. Dettwiler, M. G. Fernández, E. Tièche, K. Hahn, S. April-Monn, M. S. Dettmer, M. Kessler, S. Rottenberg, and M. Campos (2021). Validation of Immunohistochemistry for Canine Proteins Involved in Thyroid Iodine Uptake and Their Expression in Canine Follicular Cell Thyroid Carcinomas (FTCs) and FTC-Derived Organoids. Veterinary Pathology, 58(6); 1172–1180
Jin, Y., D. Shi, and L. Wang (2025). Application Value of Chemiluminescence Immunoassay in Thyroid Function Biochemical Testing. Journal of Fluorescence
Kaspar, H., K. Dettmer, W. Gronwald, and P. J. Oefner (2009). Advances in Amino Acid Analysis. Analytical and Bioanalytical Chemistry, 393(2); 445–452
Khairana, A. D., S. Fiddaroini, A. B. Wijaya, R. Rosandi, A. Sabarudin, A. Safitri, W. Riawan, D. K. Wuragil, and Aulanniam (2025). Computational Analysis of TSHb Variants (Native, Mutated and Deleted) in Interaction with TSHR: Molecular Docking and Dynamic Simulations for Mechanistic Insights into Congenital Hypothyroidism. Trends in Sciences, 22(8); 10086
Kirley, T. L. and A. B. Norman (2018). Unfolding of IgG Domains Detected by Non-Reducing SDS-PAGE. Biochemical and Biophysical Research Communications, 503(2); 944–949
Klein, L., E. A. Robey, and C.-S. Hsieh (2019). Central CD4+ T Cell Tolerance: Deletion Versus Regulatory T Cell Differentiation. Nature Reviews Immunology, 19(1); 7–18
Klubo-Gwiezdzinska, J. and L. Wartofsky (2022). Hashimoto Thyroiditis: An Evidence-Based Guide to Etiology, Diagnosis and Treatment. Polish Archives of Internal Medicine, 132(3); 16222
Le, S. N., B. T. Porebski, J. McCoey, J. Fodor, B. Riley, M. Godlewska, M. Góra, B. Czarnocka, J. P. Banga, D. E. Hoke, I. Kass, and A. M. Buckle (2015). Modelling of Thyroid Peroxidase Reveals Insights into Its Enzyme Function and Autoantigenicity. PLoS ONE, 10(12); e0142615
Lewis, S. M., A. Williams, and S. C. Eisenbarth (2019). Structure and Function of the Immune System in the Spleen. Science Immunology, 4(33); eaau6085
Liu, T., Y. Wang, X. Luo, J. Li, S. A. Reed, H. Xiao, T. S. Young, and P. G. Schultz (2016). Enhancing Protein Stability with Extended Disulfide Bonds. Proceedings of the National Academy of Sciences of the United States of America, 113(21); 5910–5915
Liu, Z., M. J. Tu, C. Zhang, J. L. Jilek, Q. Y. Zhang, and A.-M. Yu (2019). A Reliable LC-MS/MS Method for the Quantification of Natural Amino Acids in Mouse Plasma: Method Validation and Application to a Study on Amino Acid Dynamics During Hepatocellular Carcinoma Progression. Journal of Chromatography B, 1124; 72–81
Loi Julia, S.-H. and L.-H. Gam (2020). SDS-PAGE Protein Profiling of a Few Commonly Consumed Beans. Journal of Biotechnology and Bioengineering, 4(1)
Ma, S. G., Y. L. Qiu, H. Zhu, H. Liu, Q. Li, and C. M. Ji (2015). Novel Genetic Variants in the TPO Gene Cause Congenital Hypothyroidism. Scandinavian Journal of Clinical and Laboratory Investigation, 75(8); 633–637
Makaraviciute, A., C. D. Jackson, P. A. Millner, and A. Ramanaviciene (2016). Considerations in Producing Preferentially Reduced Half-Antibody Fragments. Journal of Immunological Methods, 429; 50–56
Mariam, S. H. S., R. Noorain, W. S. Tan, A. Arbakariya, and O. A. Janna (2026). Purification and Characterization of Rabbit Polyclonal Immunoglobulin G Using DEAE Ion-Exchange Chromatography and Dynamic Light Scattering. Protein Expression and Purification, 242; 106938
Marx, A., Y. Yamada, K. Simon-Keller, B. Schalke, N. Willcox, P. Ströbel, and C. A. Weis (2021). Thymus and Autoimmunity. Seminars in Immunopathology, 43(1); 45–64
McLachlan, S. M. and B. Rapoport (2017). Thyroid Autoantibodies Display Both “Original Antigenic Sin” and Epitope Spreading. Frontiers in Immunology, 8; 1845
McLeod, D. S. A. and D. S. Cooper (2012). The Incidence and Prevalence of Thyroid Autoimmunity. Endocrine, 42(2); 252–265
Miller, J. F. A. P. (2020). The Function of the Thymus and Its Impact on Modern Medicine. Science, 369(6503); eaba2429
Neto, V., S. Esteves-Ferreira, I. Inácio, M. Alves, R. Dantas, I. Almeida, J. Guimarães, T. Azevedo, and A. Nunes (2022). Metabolic Profile Characterization of Different Thyroid Nodules Using FTIR Spectroscopy: A Review. Metabolites, 12(1); 53
Noor, Z., S. B. Ahn, M. S. Baker, S. Ranganathan, and A. Mohamedali (2021). Mass Spectrometry-Based Protein Identification in Proteomics: A Review. Briefings in Bioinformatics, 22(2); 1620–1638
Nugroho, I. C., M. N. Amalia, M. R. Juniardi, E. B. Marhendra, N. A. Yanma, O. R. Adianingsih, S. Winarsih, N. Widodo, and V. Yurina (2026). Oral Immunization with Recombinant Lactococcus Lactis and Retinoic Acid Boost Immune Response in Mice. Science and Technology Indonesia, 11(1); 55–64
Panjaitan, T. F. C., S. Fiddaroini, E. Suprayitno, Aulanni’am, and Hardoko (2025). Potential Analysis of Snakehead Fish Albumin Extract (Channa striata) as Immunostimulant and Anti-Inflammatory in Indomethacin-Induced Inflammatory Bowel Disease (IBD) Rats. Science and Technology Indonesia, 10(3); 725–740
Patagar, P. M. and S. Rudrappa (2022). Autoimmune Thyroiditis in Adolescents: Two Different Presentations. Karnataka Paediatric Journal, 37; 30–34
Pedersen, I. B. and P. Laurberg (2009). Antibodies to Thyroid Peroxidase and Thyroglobulin in Iodine Deficiencies. In Comprehensive Handbook of Iodine: Nutritional, Biochemical, Pathological and Therapeutic Aspects. Elsevier, pages 575–585
Pushpagiri, N., L. Gracelyn, and S. Nagalingam (2015). Prevalence of Subclinical and Overt Hypothyroidism in Infertile Women. International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 4(6); 1733–1738
Ragusa, F., P. Fallahi, G. Elia, D. Gonnella, S. R. Paparo, C. Giusti, L. P. Churilov, S. M. Ferrari, and A. Antonelli (2019). Hashimoto’s Thyroiditis: Epidemiology, Pathogenesis, Clinic and Therapy. Best Practice & Research: Clinical Endocrinology & Metabolism, 33(6); 101367
Ramadani, D., A. B. Wijaya, A. Srihardyastutie, D. K. Wuragil, R. Rosandi, A. Rudijanto, and Aulanni’am (2024). Characterization of TPO Enzyme and Its Immunogenicity in New Zealand White Rabbits (Oryctolagus cuniculus). Jurnal Penelitian Pendidikan IPA, 10(12); 10645–10652
Raphael, I., R. R. Joern, and T. G. Forsthuber (2020). Memory CD4+ T Cells in Immunity and Autoimmune Diseases. Cells, 9(3); 531
Raphael, I., S. Nalawade, T. N. Eagar, and T. G. Forsthuber (2015). T Cell Subsets and Their Signature Cytokines in Autoimmune and Inflammatory Diseases. Cytokine, 74(1); 5–17
Rapoport, B. and S. M. McLachlan (2018). Reflections on Thyroid Autoimmunity: A Personal Overview from the Past into the Future. Hormone and Metabolic Research, 50(12); 840–852
Rehlund, A. S. G., T. S. Canoy, D. S. Nielsen, M. N. Lund, and M. M. Poojary (2025). Protein Hydrolysis for Amino Acid Analysis Revisited. Food Chemistry, 495; 146224
Rose, A., D. Funk, and R. Neiger (2016). Comparison of Refractometry and Biuret Assay for Measurement of Total Protein Concentration in Canine Abdominal and Pleural Fluid Specimens. Journal of the American Veterinary Medical Association, 248(7); 789–794
Rothschild, K. J. (2016). The Early Development and Application of FTIR Difference Spectroscopy to Membrane Proteins: A Personal Perspective. Biomedical Spectroscopy and Imaging, 5(3); 231–267
Ruterbusch, M., K. B. Pruner, L. Shehata, and M. Pepper (2020). In Vivo CD4+ T Cell Differentiation and Function: Revisiting the Th1/Th2 Paradigm. Annual Review of Immunology, 38; 705–725
Salman, M. A., A. Rabiee, A. Salman, and T. E. Ftohy (2020). Antithyroid Peroxidase Antibodies and Histopathological Outcomes in Egyptian Patients Subjected to Total Thyroidectomy for Nonmalignant Nodular Goiter. Research Square
Shawkat Kawther, R., R. Tahir Lak, and S. Mohammed Zaki Abdullah (2022). The Role of Anti-TPO as an Additional Analytical Marker in Thyroid Disease Patients in Erbil City. Diyala Journal of Medicine, 23(2); 120–130
Shehata, M., H. Saad Eldien, F. Meligy, and S. Bahaidarh (2019). The Possible Protective Role of Barley Seeds on the Spleen After Administration of Glucocorticoids in Adult Albino Rats: A Histological and Immunohistochemical Study. Journal of Microscopy and Ultrastructure, 7(4); 171–180
Siriwardhane, T., K. Krishna, V. Ranganathan, V. Jayaraman, T. Wang, K. Bei, S. Ashman, K. Rajasekaran, J. J. Rajasekaran, and H. Krishnamurthy (2019). Significance of Anti-TPO as an Early Predictive Marker in Thyroid Disease. Autoimmune Diseases, 2019; 1684074
Stebegg, M., S. D. Kumar, A. Silva-Cayetano, V. R. Fonseca, M. A. Linterman, and L. Graca (2018). Regulation of the Germinal Center Response. Frontiers in Immunology, 9; 2469
Suan, D., C. Sundling, and R. Brink (2017). Plasma Cell and Memory B Cell Differentiation from the Germinal Center. Current Opinion in Immunology, 45; 97–102
Takaba, H. and H. Takayanagi (2017). The Mechanisms of T Cell Selection in the Thymus. Trends in Immunology, 38(11); 805–816
Tater, A., A. Gupta, G. Upadhyay, A. Deshpande, R. Date, and I. Y. Tamboli (2021). In Vitro Assays for Characterization of Distinct Multiple Catalytic Activities of Thyroid Peroxidase Using LC-MS/MS. Current Research in Toxicology, 2; 19–29
Tatulian, S. A. (2019). FTIR Analysis of Proteins and Protein–Membrane Interactions. In Methods in Molecular Biology, volume 2003. Humana Press, pages 281–325
Teijaro, J. R., D. Turner, Q. Pham, E. J. Wherry, L. Lefrançois, and D. L. Farber (2011). Cutting Edge: Tissue-Retentive Lung Memory CD4 T Cells Mediate Optimal Protection to Respiratory Virus Infection. The Journal of Immunology, 187(11); 5510–5514
Tintor, K. Ninković, J. Milošević, and N. Polović (2024). Gaining Insight into Protein Structure via ATR-FTIR Spectroscopy. Vibrational Spectroscopy, 134; 103726
Trifanescu, R. A. and C. Poiana (2019). Diagnosis and Management of Hypothyroidism in Adults. In Advanced Practice in Endocrinology Nursing. Springer International Publishing, pages 581–592
Tuska, H. S. A., A. M. Hariati, A. Sanusi, G. Ciptadi, B. Rumhayati, H. Susanto, G. Residiwati, and Aulanni’am (2025). Reproductive Toxicity and Histopathological Effects of Heavy Metal Exposure in Male Nile Tilapia from the Brantas River, Indonesia. Biodiversitas, 26(10); 5169–5180
Uhlén, M., A. Bandrowski, S. Carr, A. Edwards, J. Ellenberg, E. Lundberg, D. L. Rimm, H. Rodriguez, T. Hiltke, M. Snyder, and T. Yamamoto (2016). A Proposal for Validation of Antibodies. Nature Methods, 13(10); 823–827
Valentine, K. M., D. Davini, T. J. Lawrence, G. N. Mullins, M. Manansala, M. Al-Kuhlani, J. M. Pinney, J. K. Davis, A. E. Beaudin, S. S. Sindi, D. M. Gravano, and K. K. Hoyer (2018). CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. The Journal of Immunology, 201(1); 31–40
Vancauwenberghe, T., A. Snoeckx, D. Vanbeckevoort, S. Dymarkowski, and F. M. Vanhoenacker (2015). Imaging of the Spleen: What the Clinician Needs to Know. Singapore Medical Journal, 56(3); 133–144
Villela, L. F. d. A., L. C. C. d. Freitas, M. Brandão, L. Bachmann, and R. Iwakura (2018). Characterization of Thyroid Tissue Using Infrared Spectroscopy. Archives of Head and Neck Surgery, 47(1); e0874
Wang, L., F.-S. Wang, and M. E. Gershwin (2015). Human Autoimmune Diseases: A Comprehensive Update. Journal of Internal Medicine, 278(4); 369–395
Weetman, A. P. (2021). An Update on the Pathogenesis of Hashimoto’s Thyroiditis. Journal of Endocrinological Investigation, 44(5); 883–890
Weisel, F. and M. Shlomchik (2017). Memory B Cells of Mice and Humans. Annual Review of Immunology, 35; 255–284
Williams, D. E., S. N. Le, D. E. Hoke, P. G. Chandler, M. Gora, M. Godlewska, J. P. Banga, and A. M. Buckle (2020). Structural Studies of Thyroid Peroxidase Show the Monomer Interacting with Autoantibodies in Thyroid Autoimmune Disease. Endocrinology, 161(2); bqaa016
Yadav, V., D. Dabar, A. D. Goel, M. Bairwa, A. Sood, P. Prasad, S. S. Agarwal, and S. Nandeshwar (2021). Prevalence of Hypothyroidism in Pregnant Women in India: A Meta-Analysis of Observational Studies. Journal of Thyroid Research, 2021; 5515831
Yamano, T., J. Nedjic, M. Hinterberger, M. Steinert, S. Koser, S. Pinto, N. Gerdes, E. Lutgens, N. Ishimaru, M. Busslinger, B. Brors, B. Kyewski, and L. Klein (2015). Thymic B Cells Are Licensed to Present Self Antigens for Central T Cell Tolerance Induction. Immunity, 42(6); 1048–1061
Zhang, C.-H., X.-J. Liu, Y.-F. Wang, J.-H. Lu, H.-Y. Zheng, S. Xiong, M.-Y. Zhang, and Q.-Y. Liu (2007). Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus. Virologica Sinica, 22(5); 425–431
Zhu, J. (2018). T Helper Cell Differentiation, Heterogeneity, and Plasticity. Cold Spring Harbor Perspectives in Biology, 10(10); a030338
Zhu, J., H. Yamane, and W. E. Paul (2010). Differentiation of Effector CD4+ T Cell Populations. Annual Review of Immunology, 28; 445–489
Authors
Ramadani, D., Aulanni'am, A., Baharuddin, M. F., Khairana, A. D., Fiddaroini, S., Nur, M. F., Nurmahdi, H., Riawan, W., Srihardyastutie, A., Sabarudin, A., Wuragil, D. K., Rosandi, R., Rudijanto, A., & Lubab, A. (2026). Structural Characterization and Immunogenic Evaluation of a 50 kDa Thyroid Peroxidase Fraction Isolated from Hypothyroid Patient Serum. Science and Technology Indonesia, 11(4), 1476–1496. https://doi.org/10.26554/sti.2026.11.4.1476-1496
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