The Characteristic Analysis of Caffeine Molecularly Imprinted Polymers Synthesized Using The Cooling-Heating Method, for Application as a Sensor Material

Idha Royani, Amalia Amalia, Jorena Jorena, Fitri Suryani Arsyad, Erry Koriyanti, Fiber Monado


The cooling-heating method was used to successfully synthesize molecularly imprinted polymers on caffeine. Caffeine was used as a template and mixed with chloroform solvent, methacrylic acid as a monomer, ethylene glycol dimethacrylate as a cross-linker, and benzoyl peroxide as an initiator. The solution was stirred for 15 minutes and placed in a vial. Then it was placed in a cooler with a temperature of -5C for 60 minutes and then inserted into an oven with an increasing temperature at 75C, 80C, and 85C for 3, 2 and 1 hour, respectively. Furthermore, the repeated washing process resulted in solid polymer, which was subjected to template leaching to produce polymers with specific cavities called molecularly imprinted polymers (MIP). The resulting caffeine polymer and MIP were tested using SEM, FTIR, and XRD methods. In addition, the SEM image analysis data showed 388 cavities in the polymer after template leaching, compared to the 121 cavities in the unwashed polymer. This result was supported by the FTIR spectrum analysis which showed that caffeine MIP has a higher transmittance value than the polymer. Therefore, the caffeine concentration was significantly reduced after the leaching process. The XRD spectra showed that caffeine MIP had a smaller halfmaximum diffraction peak width (FWHM) compared to the polymer. Also, the low FWHM value depicted a larger crystalline size in the caffeine MIP compared to the polymer.


Ahmad, O.S., Bedwell, T.S., Esen, C., Garcia-Cruz, A., Piletsky, S.A. (2019). Molecularly Imprinted Polymers in Electrochemical and Optical Sensors. Trends in Biotechnol. 37(3), 294-309.

Abdullah, M., Khairurrijal. (2009). Review: Karakterisasi Nanomaterial. Jurnal Nanosains & Nanoteknologi, Vol. 2 No.1.

Cormack, Mc., William, P.M.A., Hoffman, J.R. (2012). Caffeine, Energy Drinks, and Strength-Power Performance. Strength and Conditioning Journal 34(4), 11–16.

Cormack, P. A. G., Mehamod, F. S. (2013). Molecularly Imprinted Polymer Synthesis Using RAFT Polymerisation. Sains Malaysiana 42(4), 529–535.

Escott-Stump, S. (2008). Nutrition and Diagnosis-Related Care. 6th ed. Philadelphia, Pa: Lippincott Williams & Wilkins.

Farrington, K., Magner, E., Regan, F. (2006). Predicting the Performance of Molecularly Imprinted Polymers: Selective Extraction of Caffeine by Molecularly Imprinted Solid Phase Extraction. Analytica Chimica Acta 566, 60-68.

Hidayat, A.R., Sunarto. (2017). Aplikasi MIP (Molecularly Imprinted Polymer) Dengan Metanol Sebagai Ekstraktan Template dalam Sintesisnya untuk Penentuan Kadar Kafein. Jurnal Pendidikan Kimia 6(2).

Komiyama, M., Takeuchi, T., Mukawa, T., Asanuma, H. (2003). Molecular Imprinting, from Fundamentals to Applications. German: Wiley-VCH.

Koriyanti, E., Saleh, K., Monado, F., Syawali, F., Royani I. (2020). On The Effect of Ethanol Solution on Melamine Template Removal Process, Journal of Chemical Technology and Metallurgy, 55, 1, 2020, 34-39

Lavignac, N., Brain, K. R., Allender, C. J. (2006). Concentration Dependent Atrazin–Atrazin Complex Formation Promotes Selectivity in Atrazin Imprinted Polymers. Biosensors and Bioelectronics 22, 138 - 144.

Lian, H., Jun, W.X., Wu, G.H., Jun, W.Z. (2013). Preparation of Caffeine Molecularly Imprinted Polymers and Application on Solid Phase Extraction. Chinese Journal of Analytical Chemistry. 40(7), 1071-1075.

Liang, R., Ruiming, Z., Wei, Q. (2009). Potentiometric sensors basedd on moleculary imprinted for determination of melamine in milk. Sensors and acuator B 141(2), 544 – 550.

Mazzotta, E., Picca, R.A., Malitesta, C., Piletsky, S.A., Piletska, E.V. (2008). Development of a sensor prepared by entrapment of MIP particles in electrosynthesised polymer films for electrochemical detection of ephedrine. Biosens. Bioelectron. 23(7), 1152–1156.

Mirmohseni, A., Pourata, R., Shojaei, M. (2014). Application of Molecularly Imprinted Polymer for Determination of Glucose by Quartz Crystal Nanobalance Technique. Journals & Magazines IEEE Sensors Journal 14(8).

Mustaghfiroh, A.M., Mulyasuryani, A. and Andayani, U. (2019). Development of Chlorpyrifos Sensor Using Molecularly Imprinted Polymer (MIP) Polyvinyl Alcohol (PVA)-Fe3O4 as Receptor. J. Pure App. Chem. Res. 8(1).

Nurhayati, T., Yanti, Royani, I., Widayani., Khairurrijal. (2016). Synthesis and Study of Guest-Rebinding of MIP Based on MAA Prepared using Theophylline Template. Journal of Physics: Conference Series 739(012143).

Royani, I., Assaidah., Widayani., Khairurrijal. (2019). The effect of atrazine concentration on galvanic cell potential based on molecularly imprinted polymers (MIPS) and aluminium as contact electrode. Journal of Physics: Conf. Series 1282 (012029).

Royani, I., Widayani, Abdullah, M., Khairurrijal. (2014). An Atrazine Molecularly Imprinted Polymer Synthesized Using a Cooling-Heating Method with Repeated Washing: Its Physico-chemical Characteristics and Enhanced Cavities. International Journal of Electrochemical Science 9, 5651 - 5662.

Silverstein, R. M., Webster, F. X. dan Kiemle, D. J. (2006): Spectrometric Identification of Organic Compounds, John Willey & Sons. Inc, USA.

Skoog, D.A., Holler, F.J., Crouch, S.R. (2018). Principles of Instrumental Analysis. Canada: Nelson Education.

Tian, D.T., Zhou, Y.C., Xiong, L., Lu, F.T. (2015). Synthesis and Properties of Caffeine Molecularly Imprinted Polymers Based on Konjac Glucomannan. Advances in Polymer Technology 36(1), 68-76.

Wolde, T. (2014). Effects of caffeine on health and nutrition: A Review Lecturer of Nutrition. Food Science and Quality Management 30, 2224-6088.


Idha Royani (Primary Contact)
Amalia Amalia
Jorena Jorena
Fitri Suryani Arsyad
Erry Koriyanti
Fiber Monado
Royani, I., Amalia, A., Jorena, J., Arsyad, F. S. ., Koriyanti, E., & Monado, F. (2021). The Characteristic Analysis of Caffeine Molecularly Imprinted Polymers Synthesized Using The Cooling-Heating Method, for Application as a Sensor Material. Science and Technology Indonesia, 6(4), 256–260.

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