Bioactivity of Endophytic Fungi Isolates from Syzygium malaccense Twigs
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Keywords:
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Antibacterial, Antioxidant, Endophytic Fungi, Syzygium malaccense
Abstract
Searching for medicinal raw materials derived from the isolation of secondary metabolite compounds is still being carried out to cure a range of illnesses, including infections and degenerative diseases caused by bacteria and free radicals. This research used endophytic fungi extract from the twig of Syzygium malaccense. The diffusion method was used for antibacterial activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used for antioxidant activity. Chemical compound was identified by using NMR spectroscopic method. This research found 5 isolate of endophytic fungi from twig of S. malaccense (YR1 – YR5). Strong antibacterial and antioxidant activity were showed by YR5. Based on molecular identification, YR5 was Fusarium verticillioides. Pure compound prodeced by endophytic fungi F. verticilloides was naphthalene (3-methoxy-7 methylnaphthalene-1,6-diol). This finding can be used as a basis for drug development.
References
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Habisukan, U. H., E. Elfita, H. Widjajanti, and A. Setiawan (2021). Chemical Characterization of Secondary Metabolite from the Endophytic Fungus Trichordema reecei isolated from The Twig of Syzygium aqueum. Science and Technology Indonesia, 6(3); 137–143
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Ibrahim, S. R. M., E. S. Elkhayat, G. A. A. Mohamed, S. M. Fat’hi, and S. A. Ross (2016). Fusarithioamide A, a New Antimicrobial and Cytotoxic Benzamide Derivative from the Endophytic Fungus Fusarium chlamydosporium. Biochemical and Biophysical Research Communications, 479(2); 211–216
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Manias, D., A. Verma, and D. K. Soni (2020). Isolation and Characterization of Endophytes: Biochemical and Molecular Approach. In Microbial Endophytes. Elsevier, pages 1–14
Mustaqim, W. A. (2021). Syzygium malaccense (L.) Merr. & LM Perry Myrtaceae. In Ethnobotany of the Mountain Regions of Southeast Asia. Springer, pages 1041–1049
Nagarajan, D. (2019). In Vitro Antioxidant Potential of Endophytic Fungi Isolated from Enicostemma axillare (Lam.) Raynal. and Ormocarpum cochinchinense (Lour.) Merr. Journal of Pharmacognosy and Phytochemistry, 8(1); 1356–1363
Oktiansyah, R., H. Widjajanti, A. Setiawan, and Elfita (2024). Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens). Science and Technology Indonesia, 9(1); 17–27
Patel, M. and K. Patel (2019). Naphthalene Substituted Benzo [c] Coumarins: Synthesis, Characterization and Evaluation of Antibacterial Activity and Cytotoxicity. Heterocyclic Communications, 25(1); 146–151
Pessôa, M. G., B. N. Paulino, M. C. R. Mano, I. A. Neri-Numa, G. Molina, and G. M. Pastore (2017). Fusarium Species—A Promising Tool Box for Industrial Biotechnology. Applied Microbiology and Biotechnology, 101; 3493–3511
Pradeep, N., S. Anupama, K. Navya, H. Shalini, M. Idris, and U. Hampannavar (2015). Biological Removal of Phenol from Wastewaters: A Mini Review. Applied Water Science, 5(2); 105–112
Rodrigo, S., C. García-Latorre, and O. Santamaria (2021). Metabolites Produced by Fungi against Fungal Phytopathogens: Review, Implementation and Perspectives. Plants, 11(1); 81
Sabutski, Y. E., E. S. Menchinskaya, L. S. Shevchenko, E. A. Chingizova, A. R. Chingizov, R. S. Popov, V. A. Denisenko, V. V. Mikhailov, D. L. Aminin, and S. G. Polonik (2020). Synthesis and Evaluation of Antimicrobial and Cytotoxic Activity of Oxathiine-Fused Quinone-Thioglucoside Conjugates of Substituted 1, 4-Naphthoquinones. Molecules, 25(16); 3577
Salae, A.-W., C. Karalai, C. Ponglimanont, A. Kanjana-Opas, and S. Yuenyongsawad (2010). Naphthalene Derivatives from Diospyros wallichii. Canadian Journal of Chemistry, 88(9); 922–927
Savi, A., M. A. Calegari, G. C. Calegari, V. A. Q. Santos, D. Wermuth, M. A. A. da Cunha, and T. L. C. Oldoni (2020). Bioactive Compounds from Syzygium malaccense Leaves: Optimization of the Extraction Process, Biological and Chemical Characterization. Acta Scientiarum. Technology, 42(1); 1–14
Slama, H. B., A. Chenari Bouket, F. N. Alenezi, Z. Pourhassan, P. Golińska, T. Oszako, and L. Belbahri (2021). Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes. Forests, 12(12); 1784
Sondergaard, T. E., L. G. Klitgaard, S. Purup, H. Kobayashi, H. Giese, and J. L. Sørensen (2012). Estrogenic Effects of Fusarielins in Human Breast Cancer Cell Lines. Toxicology Letters, 214(3); 259–262
Syarifah, S., E. Elfita, H. Widjajanti, A. Setiawan, and A. R. Kurniawati (2021). Diversity of Endophytic Fungi from the Root Bark of Syzygium zeylanicum, and the Antibacterial Activity of Fungal Extracts, and Secondary Metabolite. Biodiversitas Journal of Biological Diversity, 22(10); 4572–458
Tamura, K., G. Stecher, and S. Kumar (2021). Mega11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38(7); 3022–3027
Tiwari, P. and H. Bae (2022). Endophytic Fungi: Key Insights, Emerging Prospects, and Challenges in Natural Product Drug Discovery. Microorganisms, 10(2); 360
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Walsh, T. J., R. T. Hayden, and D. H. Larone (2018). Larone’s Medically Important Fungi: A Guide to Identification. ASM Press
Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. CRC Press
White, J. F., K. L. Kingsley, Q. Zhang, R. Verma, N. Obi, S. Dvinskikh, M. T. Elmore, S. K. Verma, S. K. Gond, and K. P. Kowalski (2019). Review : Endophytic microbes and their potential applications in crop management. Pest Management Science, 75(10); 2558–2565
Zaher, A. M., M. A. Makboul, A. M. Moharram, B. L. Tekwani, and A. I. Calderón (2015). A New Enniatin Antibiotic from the Endophyte Fusarium tricinctum Corda. The Journal of Antibiotics, 68(3); 197–200
Alam, B., M. A. Khan, J. Gong, Y. Yuán, and W. G ¯ ong (2021). ˇ Endophytic Fungi: From Symbiosis to Secondary Metabolite Communications or Vice Versa? Frontiers in Plant Science, 12; 791033
Azam, F., S. Singh, S. L. Khokhra, and O. Prakash (2007). Synthesis of Schiff Bases of Naphtha [1, 2-d] Thiazol-2-Amine and Metal Complexes of 2-(2’-Hydroxy) Benzylideneaminonaphthothiazole As Potential Antimicrobial Agents. Journal of Zhejiang University Science B, 8(6); 446–452
Deshmukh, H. S., Y. Liu, O. R. Menkiti, J. Mei, N. Dai, C. E. O’leary, P. M. Oliver, J. K. Kolls, J. N. Weiser, and G. S. Worthen (2014). The Microbiota Regulates Neutrophil Homeostasis and Host Resistance to Escherichia coli K1 Sepsis in Neonatal Mice. Nature Medicine, 20(5); 524–530
Gustianingtyas, M., S. Herlinda, H. Hamidson, A. Setiawan, and M. Verawaty (2020). Toxicity of Entomopathogenic Fungal Culture Filtrate of Lowland and Highland Soil of South Sumatra (indonesia) against Spodoptera litura Larvae. Biodiversitas Journal of Biological Diversity, 21(5); 1839–1849
Habisukan, U., E. Elfita, H. Widjajanti, and A. Setiawan (2022). Secondary Metabolite and Antioxidant Activity of Endophytic Fungi Isolated from Syzygium aqueum Leaves Stalk. Biointerface Research in Applied Chemistry, 12(6); 7584–7595
Habisukan, U. H., E. Elfita, H. Widjajanti, and A. Setiawan (2021). Chemical Characterization of Secondary Metabolite from the Endophytic Fungus Trichordema reecei isolated from The Twig of Syzygium aqueum. Science and Technology Indonesia, 6(3); 137–143
Hapida, Y. and H. Widjajanti (2022). A Phenolic Compound from Active Extract of Endophytic Fungus Isolated from Leaf Stalk of Jambu Bol (Syzygium malaccense). International Journal Of Intelligent Systems And Applications In Engineering, 10(3); 274–280
Herlinda, S., R. A. Efendi, R. Suharjo, H. Hasbi, A. Setiawan, E. Elfita, and M. Verawaty (2020). New Emerging Entomopathogenic Fungi Isolated from Soil in South Sumatra (Indonesia) and Their Filtrate and Conidial Insecticidal Activity against Spodoptera litura. Biodiversitas Journal of Biological Diversity, 21(11); 5102–5113
Ibrahim, S. R. M., E. S. Elkhayat, G. A. A. Mohamed, S. M. Fat’hi, and S. A. Ross (2016). Fusarithioamide A, a New Antimicrobial and Cytotoxic Benzamide Derivative from the Endophytic Fungus Fusarium chlamydosporium. Biochemical and Biophysical Research Communications, 479(2); 211–216
Jigjiddorj, E.-A., A. Maidarjav, B. Byambasuren, and D. Nyamgerel (2024). Identification, Antimicrobial and Plant Growth Promoting Activities of Endophytic Fungi Associated with Cynomorium songaricum Rupr., a Traditional Medicinal Plant in Mongolia. Diversity, 16(2); 122
Kelley, C., S. Lu, A. Parhi, M. Kaul, D. S. Pilch, and E. J. LaVoie (2013). Antimicrobial Activity of Various 4-and 5-Substituted 1-Phenylnaphthalenes. European Journal of Medicinal Chemistry, 60(1); 395–409
Khan, M. A., S. Asaf, A. L. Khan, I. Ullah, S. Ali, S.-M. Kang, and I.-J. Lee (2019). Alleviation of Salt Stress Response in Soybean Plants with the Endophytic Bacterial Isolate Curtobacterium sp. SAK1. Annals of Microbiology, 69(8); 797–808
Kushwaha, P., P. L. Kashyap, P. Kuppusamy, A. K. Srivastava, and R. K. Tiwari (2020). Functional Characterization of Endophytic Bacilli from Pearl Millet (Pennisetum glaucum) and Their Possible Role in Multiple Stress Tolerance. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 154(4); 503–514
Manias, D., A. Verma, and D. K. Soni (2020). Isolation and Characterization of Endophytes: Biochemical and Molecular Approach. In Microbial Endophytes. Elsevier, pages 1–14
Mustaqim, W. A. (2021). Syzygium malaccense (L.) Merr. & LM Perry Myrtaceae. In Ethnobotany of the Mountain Regions of Southeast Asia. Springer, pages 1041–1049
Nagarajan, D. (2019). In Vitro Antioxidant Potential of Endophytic Fungi Isolated from Enicostemma axillare (Lam.) Raynal. and Ormocarpum cochinchinense (Lour.) Merr. Journal of Pharmacognosy and Phytochemistry, 8(1); 1356–1363
Oktiansyah, R., H. Widjajanti, A. Setiawan, and Elfita (2024). Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from Fruit of Sungkai (Peronema canescens). Science and Technology Indonesia, 9(1); 17–27
Patel, M. and K. Patel (2019). Naphthalene Substituted Benzo [c] Coumarins: Synthesis, Characterization and Evaluation of Antibacterial Activity and Cytotoxicity. Heterocyclic Communications, 25(1); 146–151
Pessôa, M. G., B. N. Paulino, M. C. R. Mano, I. A. Neri-Numa, G. Molina, and G. M. Pastore (2017). Fusarium Species—A Promising Tool Box for Industrial Biotechnology. Applied Microbiology and Biotechnology, 101; 3493–3511
Pradeep, N., S. Anupama, K. Navya, H. Shalini, M. Idris, and U. Hampannavar (2015). Biological Removal of Phenol from Wastewaters: A Mini Review. Applied Water Science, 5(2); 105–112
Rodrigo, S., C. García-Latorre, and O. Santamaria (2021). Metabolites Produced by Fungi against Fungal Phytopathogens: Review, Implementation and Perspectives. Plants, 11(1); 81
Sabutski, Y. E., E. S. Menchinskaya, L. S. Shevchenko, E. A. Chingizova, A. R. Chingizov, R. S. Popov, V. A. Denisenko, V. V. Mikhailov, D. L. Aminin, and S. G. Polonik (2020). Synthesis and Evaluation of Antimicrobial and Cytotoxic Activity of Oxathiine-Fused Quinone-Thioglucoside Conjugates of Substituted 1, 4-Naphthoquinones. Molecules, 25(16); 3577
Salae, A.-W., C. Karalai, C. Ponglimanont, A. Kanjana-Opas, and S. Yuenyongsawad (2010). Naphthalene Derivatives from Diospyros wallichii. Canadian Journal of Chemistry, 88(9); 922–927
Savi, A., M. A. Calegari, G. C. Calegari, V. A. Q. Santos, D. Wermuth, M. A. A. da Cunha, and T. L. C. Oldoni (2020). Bioactive Compounds from Syzygium malaccense Leaves: Optimization of the Extraction Process, Biological and Chemical Characterization. Acta Scientiarum. Technology, 42(1); 1–14
Slama, H. B., A. Chenari Bouket, F. N. Alenezi, Z. Pourhassan, P. Golińska, T. Oszako, and L. Belbahri (2021). Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes. Forests, 12(12); 1784
Sondergaard, T. E., L. G. Klitgaard, S. Purup, H. Kobayashi, H. Giese, and J. L. Sørensen (2012). Estrogenic Effects of Fusarielins in Human Breast Cancer Cell Lines. Toxicology Letters, 214(3); 259–262
Syarifah, S., E. Elfita, H. Widjajanti, A. Setiawan, and A. R. Kurniawati (2021). Diversity of Endophytic Fungi from the Root Bark of Syzygium zeylanicum, and the Antibacterial Activity of Fungal Extracts, and Secondary Metabolite. Biodiversitas Journal of Biological Diversity, 22(10); 4572–458
Tamura, K., G. Stecher, and S. Kumar (2021). Mega11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38(7); 3022–3027
Tiwari, P. and H. Bae (2022). Endophytic Fungi: Key Insights, Emerging Prospects, and Challenges in Natural Product Drug Discovery. Microorganisms, 10(2); 360
Trisuwan, K., N. Khamthong, V. Rukachaisirikul, S. Phongpaichit, S. Preedanon, and J. Sakayaroj (2010). Anthraquinone, Cyclopentanone, and Naphthoquinone Derivatives from the Sea Fan-Derived Fungi Fusarium spp. PSU-F14 and PSUF135. Journal of Natural Products, 73(9); 1507-1511
Walsh, T. J., R. T. Hayden, and D. H. Larone (2018). Larone’s Medically Important Fungi: A Guide to Identification. ASM Press
Watanabe, T. (2010). Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species. CRC Press
White, J. F., K. L. Kingsley, Q. Zhang, R. Verma, N. Obi, S. Dvinskikh, M. T. Elmore, S. K. Verma, S. K. Gond, and K. P. Kowalski (2019). Review : Endophytic microbes and their potential applications in crop management. Pest Management Science, 75(10); 2558–2565
Zaher, A. M., M. A. Makboul, A. M. Moharram, B. L. Tekwani, and A. I. Calderón (2015). A New Enniatin Antibiotic from the Endophyte Fusarium tricinctum Corda. The Journal of Antibiotics, 68(3); 197–200
Authors
Hapida, Y. ., Widjajanti, H. ., Salni, Habisukan, U. H. ., Alfarado, D. ., & Elfita. (2024). Bioactivity of Endophytic Fungi Isolates from Syzygium malaccense Twigs. Science and Technology Indonesia, 9(2), 448–456. https://doi.org/10.26554/sti.2024.9.2.448-456
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